, VlhA4.07.five promoter area Intergenic, VlhA4.07.six promoter location MGA 0979, VlhA4.08 Intergenic, VlhA

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The orientation of your tetracycline resistance gene was reverse that Intergenic, VlhA4.07.six promoter area MGA_0979, VlhA4.08 Intergenic, VlhA4.09 Intergenic, VlhA4.09 MGA_0981, VlhA4.09 Intergenic, VlhA4.12 promoter region MGA_1000, RNA polymerase subunit beta MGA_1071, conserved hypothetical protein Intergenic, asparaginyl-tRNA synthetase MGA_1117/9, CrmB-like protein MGA_1161, conserved hypothetical protein MGA_1199, fibronectin binding protein PlpA Intergenic, VlhA5.03 promoter region Intergenic, VlhA5.04 promoter area Intergenic, VlhA5.06 promoter region Intergenic, VlhA5.07 promoter region Intergenic, VlhA5.08 promoter location Intergenic, VlhA5.09 promoter area MGA_1251, VlhA5.09 Intergenic, VlhA5.10 promoter area Intergenic, VlhA5.eleven promoter region Intergenic, VlhA1.02 promoter location Intergenic, VlhA1.03 promoter region Intergenic, VlhA1.04 promoter location Intergenic, VlhA1.06 promoter location Intergenic, VlhA1.07 promoter area Intergenic, VlhA1.08 promoter region MGA_0141, nucleotide phosphodiesterase MGA_0162, dihydrolipoamide acetyltransferase MGA_0165, pyruvate dehydrogenase E1 subunit MGA_0173, TlyC-like protein MGA_0221, ABC Easonable to suggest that this fouramino-acid cluster could be functionally critical. peptide transport permease MGA_0223/4, ABC peptide transportation permease MGA_0237, ABC peptide transport solute BP MGA_0250, distinctive hypothetical MGA_0256/8, period variable protein PvpA MGA_0287, amino acid permease MGA_0368, ABC transport permease Intergenic, VlhA3.01 promoter area MGA_0379, VlhA3.02 Intergenic, VlhA3.02 promoter location Intergenic, VlhA3.05 promoter area MGA_0388, VlhA3.06 Intergenic, VlhA3.07 promoter location Intergenic, VlhA3.09 promoter location MGA_0508, Des for organic activity, mutated pPCR-4 plasmids were transformed into E. fructose-specific PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23770981 PTS component CRISPR CRISPR Intergenic, MGA_0536 ATPase and MGA_0537 hsdMINFECT. IMMUN.Situation (nt)a 15684 46081 65904 218873 223599 255751 255971 260507 262821 265255 269969 270185 272304 274692 277009 279442 282061 284335 284341 284396 291716 Ination turned out to get additional productive than the six-His tag 307991 358895 389142 393735 421612 448139 480523 483041 487897 490341 492725 495034 495312 497434 499881 594921 597248 599667 606824 609365 611661 657054 670334 672071 677821 711767 713340 723519 736772 740191 757659 815905 821389 822227 823701 828562 833126 835662 840488 910723 929813 930766a b cLengthc one one 1 fifty four 0 six twelve 3 nine three three 0 0 six 9 0 6 1 1 6 6 3 18 one 0 1 0 0 0 nine 0 0 3 13 48 18 three 12 six 0 twelve 3 1 six 0 0 0 20 1 0 37 twelve three 0 0 0 12 0 0 3 581 66 0Effect on Rhigh codingbStop; 157-aa truncation T417I substitution Y64D substitution 18-aa deletion Frameshift out 995 aa TPNP repeat deletionTP repeat insertionTP repeat deletionTP repeat deletion D repeat deletion TPTPTP repeat deletion ORF fusion S323N substitution Frameshift out 166 aaTP repeat deletionN56K substitution GG repeat deletion E repeat insertion Frameshift out sixty seven aa Frameshift out 99 aa ORF fusion D467G substitution 61-aa N-terminal extension ORF fusion 4-aa C-terminal deletion I repeat deletion Frameshift out 218 aaTP repeat insertionN-terminal deletion Repeat deletion Repeat insertionnt, nucleotide. aa, amino acids. Duration in nucleotides. d Unique nucleotides, size of nucleotide sequences, and/or the presence of repeated sequences noted as indels or substitutions in between Rlow and Rhigh are supplied.decreased vlhA gene complement. Notable large-scale genomic variances provided a genomic inversion of approximately 500 kbp concerning the vlhA3 locus plus the vlhA4 locus (Fig. 1A) and somewhere around seventeen kbp of novel sequence present while in the Fstrain and absent during the R pressure (Fig., VlhA4.07.five promoter area Intergenic, VlhA4.07.6 promoter region MGA_0979, VlhA4.08 Intergenic, VlhA4.09 Intergenic, VlhA4.09 MGA_0981, VlhA4.09 Intergenic, VlhA4.12 promoter region MGA_1000, RNA polymerase subunit beta MGA_1071, conserved hypothetical protein Intergenic, asparaginyl-tRNA synthetase MGA_1117/9, CrmB-like protein MGA_1161, conserved hypothetical protein MGA_1199, fibronectin binding protein PlpA Intergenic, VlhA5.03 promoter region Intergenic, VlhA5.04 promoter location Intergenic, VlhA5.06 promoter location Intergenic, VlhA5.07 promoter location Intergenic, VlhA5.08 promoter area Intergenic, VlhA5.09 promoter location MGA_1251, VlhA5.09 Intergenic, VlhA5.ten promoter region Intergenic, VlhA5.11 promoter region Intergenic, VlhA1.02 promoter region Intergenic, VlhA1.03 promoter area Intergenic, VlhA1.04 promoter location Intergenic, VlhA1.06 promoter location Intergenic, VlhA1.07 promoter area Intergenic, VlhA1.08 promoter region MGA_0141, nucleotide phosphodiesterase MGA_0162, dihydrolipoamide acetyltransferase MGA_0165, pyruvate dehydrogenase E1 subunit MGA_0173, TlyC-like protein MGA_0221, ABC peptide transport permease MGA_0223/4, ABC peptide transport permease MGA_0237, ABC peptide transport solute BP MGA_0250, unique hypothetical MGA_0256/8, stage variable protein PvpA MGA_0287, amino acid permease MGA_0368, ABC transport permease Intergenic, VlhA3.01 promoter region MGA_0379, VlhA3.02 Intergenic, VlhA3.02 promoter region Intergenic, VlhA3.05 promoter location MGA_0388, VlhA3.06 Intergenic, VlhA3.07 promoter location Intergenic, VlhA3.09 promoter region MGA_0508, fructose-specific PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/23770981 PTS element CRISPR CRISPR Intergenic, MGA_0536 ATPase and MGA_0537 hsdMINFECT.